Year: 2015 | Month: June | Volume 8 | Issue 2
Method Validation for Qualitative and Quantitative Analysis of Pesticide Residues in Tomato with GC-MS/MS (TQD) for Food Safety Testing
DOI:10.5958/2230-732X.2015.00053.4
Abstract:
Pesticide residues analysis in fruits and fresh vegetables is a challenge for food safety as the gap between pesticide sprays and harvests is very less in vegetables. A multi residue method was developed for qualitative and quantitative analysis of 64 pesticides (insecticides, fungicides and herbicides) using QuEChERS extraction method and GC-MS/MS (Triple Quadrupole) for analysis. Eight different concentrations of certified reference materials from 0.05 ppm to 0.30 ppm were injected in GC-MS/MS with MRM method, in six replications, and R2 ranged from 0.990-0.999 with RSD of 0.55 to 11.24. The sample preparation approach is through adoption of QuEChERS method, untreated control tomato samples were fortified with mixture of pesticides at 0.05, 0.25, 0.5 mg/kg, each is five replications, and the recovery of pesticides is in the range of 80-95%, and hence method can be used for qualitative and quantitative analysis of 64 pesticides in/on tomato for monitoring studies.
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